Changing the adjustment on the iris diaphragm changes the size of the cone of light that shines through the specimen. A smaller aperture means a smaller opening. The cone of light will have a smaller diameter.
When you adjust the iris diaphragm from big aperture to small aperture?reffering to the microscope?
This question, along with the others you have asked about microscopes simply does not make any sense. If you want answers at least form proper questions for people to answer.
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